The Global Hesperidinase Market was valued at USD 22 Million in 2023 and is projected to reach USD 27.96 Million by 2032, growing at a Compound Annual Growth Rate (CAGR) of 2.7% during the forecast period (2024–2032). This steady expansion reflects increasing demand for enzymatic solutions across pharmaceutical, nutraceutical, and cosmetic sectors seeking to enhance bioavailability of citrus-derived flavonoids.

As formulation scientists prioritize plant-based bioactives with improved solubility profiles, hesperidinase has emerged as a critical tool for converting hesperidin into more bioavailable rutinosides. This report examines the Top 10 Hesperidinase Manufacturers—biochemical innovators and specialized enzyme producers enabling next-generation applications through microbial fermentation and protein engineering advancements.

---

🔟 1. Ulcho Biochemical

Headquarters: Seoul, South Korea
Key Specialty: Ultra-pure (>99%) hesperidinase for pharmaceutical applications

As the Asia-Pacific region’s foremost enzyme producer, Ulcho Biochemical has pioneered industrial-scale hesperidinase production through optimized Aspergillus niger fermentation techniques. Their enzyme preparations are integral to flavonoid modification processes for major nutraceutical brands.

Technical Superiority:

• Proprietary strain development yielding 23% higher specific activity
• Dual-site manufacturing (Korea and China) with 80MT annual capacity
• Validated enzymatic assays for batch consistency (RSD <5%)

Download FREE Sample Report: Hesperidinase Market – View in Detailed Research Report

---

9️⃣ 2. Hangzhou Keying Chem

Headquarters: Zhejiang, China
Core Strength: Cost-optimized industrial-grade enzyme blends

This Chinese biochemical leader supplies over 35% of Asia’s bulk hesperidinase demand, specializing in enzyme systems for citrus processing and agricultural applications. Their vertical integration from raw material sourcing to downstream processing ensures competitive pricing.

Production Advantages:

• 2000L fermentation tanks with automated controls
• Formulations meeting FCC and JP pharmacopeia standards
• Custom enzyme cocktails for pectin reduction

---

8️⃣ 3. Hangzhou Fanda Chemical

Headquarters: Hangzhou, China
Key Innovation: Thermostabilized cosmetic-grade enzymes

Fanda Chemical has developed patented enzyme stabilization technology enabling hesperidinase integration into creams and serums. Their HT-200 series maintains 85% activity after 6 months at 25°C—double conventional formulations.

Market Penetration:

• Supplies 5 major European skincare brands
• First to market with cold-process compatible enzymes
• Microencapsulation protects activity in alkaline formulations

---

7️⃣ 4. Biosynth Carbosynth

Headquarters: Staad, Switzerland
Unique Offering: cGMP-certified research quantities

For pharmaceutical developers requiring regulatory-grade materials, Biosynth Carbosynth provides document-rich hesperidinase with full ICH stability data. Their enzyme reference materials are cited in 17 published pharmacokinetic studies.

Quality Systems:

• ISO 17025 accredited analytical testing
• Phase-appropriate documentation packages
• Strict -20°C冷链logistics

Download FREE Sample Report: Hesperidinase Market – View in Detailed Research Report

---

6️⃣ 5. Creative Enzymes

Headquarters: New York, USA
Specialization: FDA-supportive enzyme documentation

This US-based provider delivers hesperidinase batches with complete regulatory submission packages—including mutagenicity studies and residue documentation—accelerating IND filings for novel flavonoid drugs.

Services Include:

• 21 CFR Part 11 compliant manufacturing
• DMF cross-referencing support
• Extractables/leachables profiling

---

5️⃣ 6. Xi’an Lyphar Biotech

Headquarters: Shaanxi, China
Innovation Driver: Custom activity-tuned formulations

Lyphar’s application-tailored hesperidinase blends optimize performance for specific use cases—whether rapid flavonoid hydrolysis in batch processing or controlled release in supplements. Their HPL-9 variant shows 40% faster kinetics than standard preparations.

Technical Service:

• Application testing protocols
• Substrate specificity optimization
• Co-enzyme blending services

---

4️⃣ 7. Huayuan Shengtai Biological

Headquarters: Xi’an, China
Competitive Edge: Academic-industry research collaborations

This biotech firm bridges fundamental enzyme research with commercial production, working with 8 Chinese universities to develop next-generation hesperidinases. Their HT-3 mutant demonstrates unprecedented stability in acidic environments.

R&D Investments:

• 16 patent families covering engineered variants
• High-throughput screening platform
• Directed evolution capabilities

---

3️⃣ 8. ChemFaces Biochemical

Headquarters: Wuhan, China
Value Proposition: Analytical reference standards

While not a bulk producer, ChemFaces sets industry benchmarks with ultra-pure (>99.5%) hesperidinase certified reference materials used by regulatory agencies and quality control laboratories worldwide.

Quality Leadership:

• Third-party validated purity methods
• Stable isotope-labeled internal standards
• Forced degradation studies included

---

2️⃣ 9. BioCrick Biotech

Headquarters: Chengdu, China
Technical Focus: Extreme-condition enzymes

BioCrick’s bioinformatics-driven protein engineering has produced hesperidinase variants functioning optimally at temperatures up to 65°C and pH ranges from 3.0-8.5—dramatically expanding potential industrial applications.

Breakthroughs Include:

• Heat-stable CR-7 variant (patent pending)
• Organic solvent-tolerant OST series
• Metal-ion resistant formulations

---

1️⃣ 10. Biovet

Headquarters: Jena, Germany
Emerging Player: Animal-free production system

This European newcomer has developed a proprietary plant-cell expression platform yielding vegan-certified hesperidinase, appealing to clean-label manufacturers. Initial batches show competitive activity levels with improved allergen profiles.

Sustainability Advantages:

• 80% lower water usage vs microbial fermentation
• Carbon-neutral production commitment
• Non-GMO certification achieved

Get Full Report Here: Hesperidinase Market – View in Detailed Research Report

---

🔬 Future Outlook: Enzymatic Innovation Frontier

The hesperidinase landscape is transitioning from commoditized enzymes to high-value specialty solutions. As analytical techniques improve and protein engineering costs decline, we anticipate three key developments:

📈 Emerging Application Areas:

• Precision fermentation for rare flavonoid derivatives
• Immobilized enzyme systems for continuous processing
• Synergistic blends with other glycosidases
• FDA-approved drug delivery enhancers

The profiled companies are not merely enzyme suppliers—they’re enabling technology partners driving tangible formulation breakthroughs across multiple industries.

Get Full Report Here: Hesperidinase Market – View in Detailed Research Report